Gabriel Rosangkima and Surya Bali Prasad
Keywords: Dillenia pentagyna – antimutagenicity - benzo[a]pyrene – cisplatin.
Abstract: Modulatory effect of methanol extract of stem bark of Dillenia pentagyna (DPE) was evaluated against mutagenicity induced by cisplatin (CIS) and benzo[a]pyrene (B[a]P) in the mouse using bone marrow chromosomal aberration, micronucleus and sperm abnormality as mutagenicity parameters. In one group of experiment, animals received a single dose (20mg/kg body weight) of DPE through intraperitoneal injection (i.p) followed by cisplatin or benzo[a]pyrene treatment. In the other groups, three doses of DPE (20, 50 and 100mg/kg b. wt/day) were given through diet for seven consecutive days prior to a single treatment with cisplatin or benzo[a]pyrene. The animals from different treatment groups were used for the study of chromosomal aberration (CA), micronucleus (MN) and sperm abnormality assays. The result of present study shows that a single treatment with DPE did not show significant changes in the incidence of chromosomal aberration, micronucleus and sperm abnormality induced by CIS and B[a]P. However, pretreatment with DPE for seven consecutive days dose dependently reduced CIS and B[a]P-induced mutagenicity suggesting the protective role of D. pentagyna on CIS and B[a]P mutagenic potentials.
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