M Zaheer Khan, Ghazala Yasmeen, SNH Naqvi and Aisha Perveen
Keywords: Euphlyctis cyanophlyctis, cholinesterase, alkaline phosphatase, chlorpyrifos, dathrin.
Abstract: Globally many scientists believe that several common pesticides already exist at levels capable of killing amphibians in the earth. The aquatic biota may be harmed by pesticide-contaminated water. The present study was done to investigate the effects of two pesticide groups organophosphate and pyrethroid, on the activity of cholinesterase (ChE) and alkaline phosphatase (ALP) in the liver, kidney and brain tissue of Euphlyctis cyanophlyctis. LD50 of each pesticide was determined before the selection of final concentrations of both pesticides. The frogs were treated by two concentrations of chlorpyrifos i.e. 2 and 4%. The effect of these two concentrations on ChE activity in the liver, kidney and brain was estimated. According to results it was decreased upto 30.0 and 45.0% in liver (F2,6=116.90, P=0.001), 20.0 and 50.0% (F2,6=8.99, P=0.016) in kidney and 33.33 and 55.55 % (F2,6=63.96, P=0.001) in the brain, respectively. Under the effect of two concentrations of Dathrin i.e. 0.04 and 0.08% the ChE activity in the liver was decreased upto 15.0 and 45.0% (F2,6=14.90, P=0.005), in the kidney it was decreased upto 20.0 and 40.0 % (F2,6=7.30, P=0.025), while in the brain the activity decreased upto 22.0 and 44.0 %, respectively (F2,6=6.80, P=0.029). The effects of same concentrations of both pesticides were also observed on alkaline phosphatase (ALP) activity in liver, kidney and brain of E. cyanophlyctis. In the case of chlorpyrifos it was decreased upto 25.0 and 50.0 % (F2,6=7.00, P=0.027), in kidney ALP activity decreased upto 33.33 and 50.0 % (F2,6=1.98, P=0.219), while in the brain it decreased upto 22.22 and 44.44 %, respectively (F2,6=1.89, P= 0.231). The effect of Dathrin on ALP in liver it was decreased upto 37.50 and 50.0 % (F2,6=73.0, P= 0.001), in the kidney the activity was decreased upto 16.66 and 50.0% (F2,6=2.02, P=0.214), while in the brain it was decreased upto 44.44 and 55.55%, respectively (F2,6=1.98, P=0.219).
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