Ibtisam M Ababutain, Zeinab K Abdul Aziz and Nijla A. AL-Meshhen
Keywords: Streptomyces sp., purification, lincomycin.
Abstract: The most potent actinomycete isolates which was previously identified as Streptomyces sp. MS-266 Dm4 was selected for the biosynthesis of the active metabolite having biodiversal activities. The active metabolite was extracted by diethyl ether at pH 7.0. The organic phase was collected and evaporated under reduced pressure using a rotary evaporator. The extract was concentrated and treated with petroleum ether (b.p. 60-80ºC) for precipitation process, where only one fraction was obtained in the form of yellowish brown viscous texture. The purification process was performed using both thin layer (TLC) and column chromatography (CC) techniques. The active compound under study was tested for its physicochemical characteristics, where the results revealed that the compound melting point is 155oC; and soluble in chloroform, n-butanol, methanol, acetone, ethanol, ethyl acetate and isopropyl alcohol but insoluble in petroleum ether, hexane and water. The elemental analysis of the active compound suggested the empirical formula of: (C10 H20 N2 O16). The spectroscopic characteristics of active compound revealed the presence of the maximum absorption peak in UV at 269 nm, infrared absorption spectrum represented by nine peaks in addition to Mass- spectrum suggests the molecular weight of the active compound as 447 Dalton. The purified antimicrobial agent was suggestive of being belonging to Lincomycin antibiotic. The Minimum Inhibitory Concentration (MIC) of the antimicrobial agent was also determined which was found to have a bacteriostatic activity.
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