Akinboro Adetayo, Olaniyi Temitope Adedosu, Akintola Adebola Olayemi, Gbadebo Emmanuel Adeleke, Badmus Jelili Abiodun, Omolara Omowumi Babalola and Babatunde Adetunji Ayodele
Keywords: Lannae egregia, haematological disorder, attenuates, ethyl acetate extract, dyslipidaemia, Anti-oxidative.
Abstract: Methotrexate is a known chemotherapeutic agent with high degree of potency accumulating in tissues inducing varying degrees of toxicities. This study investigated the haematological disorder and dyslipidaemia associated with methotrexate toxicities and the effects of ethyl acetate extract of Lannae egregia (EELE) leaves, a local anticancer medicinal plant in rats. Forty-eight male Wistar rats averagely weighing 150 g were randomly selected into six groups : group A (distilled water only), group B ( 2.5 mg/kg.bw of methotrexate only for 21days at 7days interval), group C (pre-treated with 100 mg/kg.bw of EELE daily and 2.5mg/kg.bw of methotrexate at 7days interval), group D (pre-treated with 100 mg/kg.bw of ascorbic acid daily and 2.5mg/kg.bw of methotrexate at 7days interval), while group E and F were administered 100 mg/kg.bw of EELE and 100 mg/kg.bw of ascorbic acid daily respectively. Administration was done orally in 0.1 ml solution for 35 days. Plasma total protein levels, haematological parameters and lipid profile were determinates via quality methods. Results showed that group B, elicit significant (p<0.05) decreases in total protein levels, red blood cell count, haemoglobin concentrations, packed cell volume and high-density lipoprotein-cholesterol levels with corresponding significant (p<0.05) increases in white blood cell count, lymphocyte, platelets, triglyceride and cholesterol levels in the plasma. However, combined treatment with methotrexate and EELE (group C) as well as rats treated with methotrexate and ascorbic acid (group D) showed ameliorative effect following metabolic alterations associated with methotrexate administration as these parameters were reversed significantly with greater effects observed in rats treated with extract only (group E) and ascorbic acid only (group F). Results are indication of haematological disorder and alterations of lipid metabolism by methotrexate, while EELE attenuates these effects via possible anti oxidative effect that compared favourably with ascorbic acid. It also showed synergistic effect with ascorbic acid in attenuating methotrexate toxicity.
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