Sherin K Sheir, Omayma, A M. Maghraby , Azza H. Mohamed, Gamalat Y. Osman and Sanya A. Al-Qormuti
Keywords: Nagella sativa, immunomodulation, granuloma, schistosomiasis
Abstract: Schistosomiasis is one of the world’s most prevalent parasitic diseases and cause of morbidity and mortality. The present study was designed to investigate the immune mechanisms possibly involved in the amelioration of histopathological changes in livers of Schistosoma mansoni infected mice treated with Nigella sativa oil combined with artemether (ART) or praziquantel (PZQ). Hitological alterations of liver and granulomas diameter were recorded. Total serum immunoglobulin G (IgG) and cytokine profils; IL-2, IL-12 and TNF-? were studied. The levels of total serum IgG after treatment with N. sativa or ART or PZQ of non-infected mice recorded a significant decrease compared to normal control group. While N. sativa combined with ART or PZQ of infected groups showed significant increase in total IgG (684.24 ± 3.03, 647.42 ± 2.21 and 708.50 ± 18.06, respectively) compared to infected lab control group (570.84 ± 6.55). Effect of N. sativa oil on cytokines IL-2 and TNF-? of non-infected and infected mice showed significant increase (P<0.001, Kruskal wallis). While treatment with ART of non-infected showed significant decrease in IL-2 and 12 and increase in TNF-?. PZQ treatment showed significant increase in IL-2 and decrease in IL-12 compared to normal control group. Effect of N. sativa oil combined with ART or PZQ showed significant increase on cytokines (IL-2, IL-12 and TNF-?) compared to infected control group. Histological observation of liver tissue of infected and non-infected mice treated with N. sativa combined with ART or PZQ showed some improvement in histological damages in all treated groups. The present data recorded that N. sativa combined with ART or PZQ recorded a significant decrease in granuloma diameter (35.42 % and 32.23 %), when compared to infected control group. It can be concluded that N. sativa is a promising adjuvant with antihelminthic drugs in the treatment of schistosomiasis.
[View Complete Article]