PHARMACOLOGICAL, BIOLOGICAL STUDY AND GC/MS ANALYSIS OF THE ESSENTIAL OIL OF THE AERIAL PARTS AND THE ALCOHOL SOLUBLE FRACTION OF THE N. HEXANE EXTRACT OF THE FLOWERS OF REICHARDIA TINGITANA L. Page No: 3167-3175

Taha El Alfy, Mona E El Tantawy, Amira Abdel Motaal, Fatma El Zahraa Gamal

Keywords: Reichardia tingitana L., Asteraceae, essential oils.

Abstract: The essential oil of Reichardia tingitana L. of the aerial parts (stems and leaves) and flowers extract were obtained by hydrodistillation and extraction methods, respectively, then were analyzed by Gas Chromotography-Mass Spectrometry (GC / MS). Twenty-two components comprising 93.446% of the oil of the aerial parts and forty-five components comprising 98.056% of flowers extract were identified. The major components of aerial parts oil were cinnamaldehyde (29.78%), and undecane (12.99%). The flowers extract contained lanoleic acid ethyl ester (34.146%), hexadecanoic acid ethyl ester (20.6%) and linoleic acid methyl ester (8.05%) as the major components. Both oil and extract were examined for their anti-microbial activities against gram positive, gram negative bacteria and fungi. The results revealed the potent anti-microbial activity of the oil. Both oil and extract were studied for their cytotoxic activities against three different human cell lines. The oil showed potent cytotoxic activity against tumor cells.



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