Kolawole OT and Akanji MA
Keywords: Newbouldia laevis, inflammation, antioxidant, diabetes, cytokines.
Abstract: This study was designed to evaluate anti-inflammatory and antioxidant potentials of ethanolic extract of leaves of Newbouldia laevis in diabetic rats. Diabetes was induced in rats by intravenous injection of freshly prepared solution of streptozotocin (60 mg/kg body weight). Diabetic rats were then treated with extract of the leaves of N. laevis (500 mg/kg body weight) for 28 days after which serum levels of tumor necrosis factor alpha (TNF-?) and interleukin -1 beta (IL-1?) were estimated using ELISA kit while serum concentration of nitric oxide (NO) was determined by Griess assay. The activities of catalase (CAT), reduced glutathione (GSH), glutathione peroxidase (GPx) and superoxide dismutase (SOD) were also estimated. Free radical scavenging activity of the extract was measured by decrease in the absorbance of methanol solution of 1,1-diphenyl-2-picrylhydrazyl (DPPH). The extract significantly reduced (P < 0.05) serum levels of nitric oxide, IL-1? as well as TNF-? in diabetic rats. The activities of CAT, GSH, GPx and SOD were significantly increased (P < 0.05) in treated diabetic rats compared to diabetic control. The extract also possesses free radical scavenging activity against DPPH with IC50 of 7.2 ?g/ml. The study showed that ethanolic extract of N. laevis leaves possesses anti-inflammatory and antioxidant properties in streptozotocin-induced diabetic rats.
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