DETECTION OF SALMONELLA TYPHI ANTIGENS ON THE SURFACE OF MOUSE PERITONEAL MACROPHAGES USING FLUORESCENT TAGGED ANTIBODIES Page No: 2211-2215

Deepika Sidhu, Sandeep Kumar, Gulshan Kumar and HG Bramhne

Keywords: S. typhi antigen, mouse peritoneal macrophages, phagocytosis, FITC tagged antibodies.

Abstract: To see the possibilities of detecting Salmonella typhi antigens on Macrophage surface, mouse peritoneal macrophages were infected with S. typhi in vitro and the macrophages were examined for morphological changes and expression of S. typhi surface antigens on the macrophage surfaces, using FITC tagged specific antibodies. The study showed positive results as fluorescence could be observed on the macrophage surfaces, however, the intensity of fluorescence was low.



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