Ibtisam M Ababutain, Zeinab K Abdul Aziz and Nijla A AL-Meshhen
Keywords: Actinomycetes, diversity, biocontrol, identification, streptomyces.
Abstract: The present study began with the isolation of 60 actinomycetes isolates from soil samples collected from different selected locations of Saudi Arabia. The purified actinomycetes isolates were subjected for screening program of antimicrobial and insecticidal activities. Isolate No. 4 isolated from Dammam governorate was found to be the most active actinomycetes isolate in which it produces an active substance belonging to lincomycin antibiotic against Gram positive, Gram negative bacteria and insects. The most active actinomycetes isolates No. 4 was selected for further studies concerning their identification. Morphological, physiological and phylogenetic analysis (16S rRNA); in addition to biochemical studies and culture characteristics as well as the chemical analysis of the cell wall, were carried out for the isolate under study. Based on the phenotypic and genotypic accumulated characteristics of the most active actinomycetes isolate and consulting the recommended International Key’s of Bergey’s Manual for identification of actinomycetes, it was found that this isolate matched with Streptomyces sp. MS-266 and was given the name Streptomyces sp. MS-266 Dm4.
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