Origanti D Rakesh,, Rajiv Pathak, Atlar Singh Dhaker, Rajesh Arora, Raj Kumar, R. Rajaram and Hemant K Gautam
Keywords: Antibacterial activity, antioxidant activity, gamma irradiation, phylogenetic analyses.
Abstract: With an objective to find new marine-derived antibiotics and antioxidants, marine bacteria were isolated from sediment samples collected from depths of 556 m and 698 m in Laccadive Sea of Indian Ocean. The antibacterial and antioxidant activity of these bacteria was studied. Preliminary assays revealed that none of the isolates possess antibacterial activity. However, exposure to gamma radiation resulted in synthesis of metabolites in one of the strain leading to the observation of antibacterial effect. Parallel to this, all the strains were evaluated for their antioxidant activity. The same strain, which exhibited antibacterial activity after gamma-irradiation, showed potent antioxidant activity. The extract which showed biological activity was studied by thin layer chromatography studies. The isolated strain was characterised to be Bacillus sonorensis based on phylogenetic analyses using molecular biological techniques.
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