SC Roy and A Chattopadhyaya
Keywords: Bacteria, Alkaline protease, detergent compatibility, 16s rDNA sequencing.
Abstract: Five alkaline protease producing bacteria were isolated from the soil of mango orchards. The total protein was estimated and protease activity was measured against casein in 0.05 M buffer (carbonate: bicarbonate) at different temperature and pH. Alkaline proteases from all five isolates were thermostable and found to retain almost full activity after 1h incubation at 40°C. The protease exhibited good stability in presence of some locally available commercial detergents like Surf Excel, Ariel, Wheel, Rin, Sunlight and Tide and found to retain almost 50% activity after 10 min incubation at 35°C. Indicating that these enzymes could be used in detergent industries. One of the five isolates was identified as Pseudomonas aeruginosa [MTCC 7807] by 16s rDNA gene sequence analysis. Morphologically they are rod shaped revealed by scanning electron microscopy.
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