SC Roy and BN Chakraborty
Keywords: TA-cloning, tea, PR-proteins, Chitinase gene, pGEMT vector, bioinformatics.
Abstract: Plant chitinases belong to a family of pathogenesis-related (PR) proteins, which are over-expressed by plants in response to a pathogen attack. Chitinases catalyze the hydrolysis of the ?-1, 4 linked N-acetylglucosamine polymers that form chitin chains, a major component of fungal cell walls. Chitinase gene specific DNA fragment from tea plant (Camellia sinensis) has been amplified in PCR reaction using gene specific primers. The PCR product of 366bp is cloned into TA cloning vector (pGEMT Easy vector) and sequenced bi-directionally. The nucleotide sequence information of 366bp has been analyzed through the different BLAST programme of bioinformatics algorithm for its molecular analysis in order to obtain genetic information carried by the DNA fragment. The chitinase gene specific fragment of 366bp of tea is deposited into the GenBank of NCBI (Accession # EU373553).
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