M Zaheer Khan and Ghazala Yasmeen
Keywords: Euphlyctis cyanophlyctis, cholinesterase, alkaline phosphatase, sandaphos, ?-cypermethrin.
Abstract: The present study was conducted to determine the effects of exposure of two pesticide groups organophosphate and pyrethroid on the activity of enzymes cholinesterase (ChE) and alkaline phosphatase (ALP) in liver, kidney and brain tissue of Euphlyctis cyanophlyctis. LD50 of each pesticide was determined. The frogs were treated by 5 and 10% of Sandaphos. The results showed decrease in ChE activity in the liver, kidney and brain upto 10.0 and 15.0% (F2,6=5.23, P=0.048) in liver, 10.0 and 50.0% (F2,6=7.00, P=0.027) in kidney, and 55.55 and 66.66% (F2,6=87.17, P=0.001) in the brain, respectively. Under the treatment of same concentrations of ?-cypermethrin the ChE activity decreased upto 20.0 and 30.0% (F2,6=13.28, P=0.006) in liver, 30.0 and 40.0% (F2,6=6.80, P=0.029) in kidney and 33.33 and 44.44% (F2,6=22.99, P=0.002) in the brain, respectively. The effects of same concentrations of both pesticides were also observed on alkaline phosphatase (ALP) activity in the liver, kidney and brain of E. cyanophlyctis. In case of sandaphos it decreased upto 50.0 and 62.50% (F2,6=91.00, P=0.001) in liver, 16.66 and 33.33 % (F2,6=1.31, P=0.337) in kidney, and 11.11 and 33.33 % (F2,6=2.25, P=0.186) in the brain, respectively, while under the treatment of same concentrations of ?-cypermethrin the ALP activity in the liver decreased upto 12.5 and 25.0% (F2,6=1.19, P=0.368), 16.66 and 33.33% (F2,6=1.66, P=0.267) in kidney, and 22.22 and 55.55 % (F2,6=1.92, P=0.227) in the brain, respectively.
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