Abstract: Enzyme catalyzed synthesis of zero generation (G0) polyamidoamine (PAMAM) dendrimer through Michael Addition
has been studied in organic solvents. Lipase from Candida antarctica has been used for synthesis of G0 PAMAM
dendrimer in ten different organic solvents. Initial rate of the reaction has been correlated with solvent properties such as
hydrophobicity, water solubility, polarizability, dielectric constant, electron pair acceptance and donation index, etc. It is
observed that there are good relationships of initial rate with solvent properties. Activity of the reaction has been
correlated with initial rate and calculated the value of specific constant (Ksp) an intrinsic specific constant (intKsp).
Solvent polarizability has been correlated with Ksp andintKsp and observed a good relationship with 0.97 R2 value. Effect
of enzyme inhibition has also been observed for the reaction which is found to be uncompetitive in nature. The values of
kinetic parameters are calculated for the reaction and reported. The enzyme remains its activity up to second cycle.