THE HISTOLOGY OF EPIPHYSEAL PLATE OF DEVELOPING THIGH BONE OF FAYOUMI UNDER THE INDUCED EFFECT OF NICOTINE AND GREEN TEA (CAMELLIA SINENSIS Page No: 4549-4552

By: Maryam Shan, Shazia Imran and Hina Shan

Keywords: Incubation, femur, nicotine, hypertrophy zone.

Abstract: Cigarette smoking has become one of the greatest developing health hazard in young people and World Health Organization showed figures regarding million tobacco-related deaths every year. The present study was conducted during the period of November 2011 - November 2012 to assessing the induced adverse effects of nicotine. Simple Random Sampling technique was used. Freshly laid fertilized eggs of Fayoumi species were selected at zero hour of incubation. Hatchery was used for placing eggs at 37.50C. The proper ventilation and relative humidity of 75% was maintained. Four groups were made each group was comprising of ten numbers of eggs. Control group G1 was given normal saline, experimental group G2 was given Camellia sinensis extract, group G3 was given 0.0001% nicotine solution, group G4 was given both 0.0001% nicotine solution and green tea extract. Double exposure of doses was given. First exposure was at 48 hours of incubation and second at 48 hours of hatching (post natal dose). At the age of one month chicks were sacrificed, thigh bones were collected dissected and duly labeled tissue cassettes and slides were made to observe the height of hypertrophy zone. Experimental groups when compared with each other such as, G2 in comparison with G3 and G4 showed significant results with p values (0.004) and (0.000), respectively. Comparison of G3 and G4 with each other showed statistically significant result with p value (0.000). Based present study, we conclude that nicotine, one of the constituent of cigarette smoke causes toxic effects on the developing thigh bone of chick.



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