EXTENT OF FISH AND MUSSEL SPECIES CONTAMINATED BY ENDOCRINE DISRUPTING CHEMICALS (EDCS) IN KARACHI COSTAL AREAS: ASSESSMENT USING AN IN-VITRO YEAST ESTROGENIC SCREENING (YES) TEST Page No: 4067-4077

By: M Zaheer Khan, Farhana Ali, Alvin Louie, Afsheen Zehra, Karim Gabol, Roohi Kanwal, Ghazala Yasmeen, M Asif Iqbal, Uzma Manzoor, Ubaid Ullah and Francis CP Law

Keywords: Karachi, coastal areas, YES bioassay, Engraulis purava, Mytilus

Abstract: The objective of the present study was to screen for Endocrine Disrupting Chemicals (EDCs) contamination in fish (Engraulis purava) and mussel (Mytilus) in 10 selected areas of Karachi coast. After the baseline survey, we selected Paradise Point, Hawks Bay, Sandspit, Manora, Baba Island, and Korangi/Phitti Creek to collect fish samples where as Buleji point 1 & point 2, Paradise point 1 & point 2, and Manora point 1 & point 2 were selected for mussel samples. The YES bioassay was used in the present study to screen marine fish and mussel for EDC. According to findings the most contaminated area is Baba Island in which the Anchovy contained 95.78ngE2 equivalents/g wet weight of fish tissue whereas the anchovy collected from Sandspit contained 20.70ng/g of fish tissue. The second most polluted area is Korangi/ Phitti Creek where the fish samples contain an average of 77.19ng/g, ww. In the mussel samples, Buleji point 1 area contains an average of 875.23ng/g, ww, and most polluted area for mussel as compared to other study areas. Further investigation such as chemical analysis has been recommended for identification of chemicals



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